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Professor Chong FANG shares research on Femtosecond Stimulated Raman Spectroscopy

Liping WEN, Yiqing CHEN     2023-08-11

On August 9, 2023, Prof. Chong FANG from  Oregon State University was invited to the 124th Science Lecture in the College of Science. He gave a lecture themed “Capturing Molecular Movies of Fluorescent Proteins and Materials with Ultrafast Stimulated Raman Spectroscopy”, which was chaired by Prof. Ruijun TIAN of the Department of Chemistry, SUSTech.

Dr. Chong FANG is a professor in the Department of Chemistry at Oregon State University, where he has significantly contributed to the field of Femtosecond Stimulated Raman Spectroscopy (FSRS) and two-dimensional infrared (2D-IR) studies. In his recent presentation, Prof. FANG elucidated the fundamental concepts of using ultrafast lasers to probe structural changes at the molecular level, while also discussing his team's recent research endeavors.


To examine the dynamics of molecules and proteins, Prof. FANG has developed the FSRS system, which encompasses three crucial processes in Raman spectroscopy. Initially, a blue laser (fs) is employed to elevate the molecules into an excited state. Subsequently, a green laser (ps) acts as the Raman pump, initiating the Raman process. Finally, a red laser (fs) serves as the Raman probe to obtain the energy diagram. FSRS utilizes the heterodyne detection method to measure signals in the detection direction and acquire corresponding spectra. This technique enables the investigation of vibrational dynamics in both the electronic ground state and excited states of molecules, including homotopic isomerization reactions. Notably, FSRS can be applied even to molecules with strong fluorescence backgrounds.

Employing FSRS, Prof. FANG and his team have investigated various aspects, including the vibrational fluorescence mechanisms of GFP chromophores and the ultrafast excited-state proton transfer processes of conformationally locked and fluorinated super-acids. Additionally, they have developed spectral probes with enhanced redness, brightness, and greater Stark shift. Furthermore, FSRS has found practical applications in molecular design and has guided the synthesis of luminescent proteins and materials. Prof. FANG envisions the extensive utilization of FSRS across the realms of biophysical chemistry, materials synthesis, and industrial production.


During the interactive Q&A session, both students and faculty members enthusiastically asked questions about the femtosecond stimulated Raman spectroscopy system, the fluorescence mechanism of fluorescent proteins, and the dynamics processes involved. Prof. FANG patiently and comprehensively answered each question in detail.



In conclusion, Academician Qiang XU and Professor Ruijun TIAN handed an honorary certificate to Prof. Chong Fang.